In general experiments, we usually store uncommonly used cells in liquid nitrogen, but store commonly used cells in freezer. Generally speaking Most of the cells can be stored at -80 degrees for three months with the addition of cryopreservation medium,At the same time, the survival rate can generally be maintained above 80%. The survival rate of the cells will decrease if stored over 6 months in -80 freezer, and the activity after loading will also be very low, So  it is recommended to store cells in liquid nitrogen if the cells will not be used in three months