As an professional researcher, the cell culture is our normal work, and very step are important. we will not get good data even if one mistake.

1、Select cells for cryopreservation

The best time to cryopreserved cells is when they are in optimal condition and near the end of logarithmic growth phase.

2、Collect cells

We should collect the cells that will be freezed in appropriate method depends on different cell type. Freezing cells and slow dow the cell metabolism.

3、Storage cells

The PP material cryovials with screw caps are the most widely used containers for cell cryopreservation. and choose appropriate cyoprotectants can reduce the damage to cooling cells.

4、Freezing cells

Freezing cells in a  fixed cooling rate so that to reduce damages to cells caused by cooling too quickly or too slowly. now the Isopropyl alcohol- free cell freezing container will be a better choice for our experiment, it can ensure the cooling rate at -1℃/minute, also there is no risk of chemical reagent contamination because without isopropyl alcohol, and we can use it againg and again and again, no need any reagent added. the cell freezing container can be stored in any dry place, it is very convenient and environmentally frendly.

more detaily welcome to visit : https://www.sdowbio.com/productinfo/674662.html or contact us directly via email cellfreezing@sdowbio.com

5、Preservate cells

When the cells have been freezed, we should transfer them to suitable environment to store in dry ice container or liquid nitrogen tank which can keep the temperatue below -130℃。

6、Thawing cells

Thawing cells must fast or will damage them.for most kinds of cells,  usually thawing cells about 60s~90s in 37 ℃ is good.

 

Generally speaking, most of freezed cell will get normal vitality when the cell medium is changed within thawing 6~8 hours , and the cryoprotectant is removed.